Sonpatha (Oroxylum indicum (L.) Vent.) is a threatened medicinal tree species [1,2] belonging to family Bignoniaceae. It is valued for its antimicrobial, antiarthritic, anticancerous and antihepatitic properties possessed by its various parts. Root extract of this tree has been used for long in ayurvedic preparations like Dashmularisht and Chyawanprash [3,4].This tree possesses a flavonoid viz. Baicalein used to check proliferation of human breast cancer cell line MDA - MB - 435 [5]. Sonpatha grows in India, Sri Lanka, South China, Celebes, Philippines and Malaysia[6,7]. In India, it is distributed throughout the country up to an altitude of 1200 m and found mainly in ravine and moist places in the forests [8].Owing to indiscriminate collection, over exploitation and uprooting of whole plants with roots, this valuable tree has become vulnerable in different states of India like Karnataka, Andhra Pradesh, Kerala, Maharastra, M.P. and Chhatisgarh [9,10]. Hence research towards mass multiplication, conservation and higher production of the active compound under in vitro culture conditions is essential [11]. Few reports are available on the in vitro regeneration of the species [12,13]. Optimum factors influencing growth and morphogenesis vary with the genotype and types of explants used for micropropagation. [14]. Murashige and Skoog (MS) medium with a high content of nitrate, ammonium and potassium is of widespread use in the successful culture of a wide variety of plants. Sometimes it requires supplementation of additional substances in the medium.
Application of additives is adapted to the cultural needs[15] i.e. objectives of the experimental studies like micropropagation, regeneration, cytodifferentiation, androgenesis, biosynthesis of secondary metabolites and biotransformation of cells as well as
the particular plant species taken. In this chapter the importance of some additives like activated charcoal (AC), casein hydrolysate (CH), coconut milk (CM) & silver nitrate (AgNO3) & their impact on the direct & indirect in vitro multiplication of a threatened medicinal tree Sonpatha (Oroxylum indicum) is emphasized.
No comments:
Post a Comment