In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants

Bacopa monnieri, a traditional Indian medicinal plant with high commercial
importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory
and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic
ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique
for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro
propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best
results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments
were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without
any plant growth hormone was used as control. Further multiplication was obtained on MS
medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was
observed by IBA when incorporated in MS at different concentrations (0.1 - 0.3 mg/l) and
0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70%
of the rooted shoots survived during hardening.

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